Characterize the Pre-catalytic State of CRISPR/Cas9
| dc.contributor.author | Liu, Jin | |
| dc.creator | Chen, Xiongping | |
| dc.date.accessioned | 2020-12-13T17:16:28Z | |
| dc.date.available | 2020-12-13T17:16:28Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Purpose: CRISPR-Cas9 has been widely used as a gene-editing tool, but its catalytic mechanism remains elusive. Cas9 has two catalytic domains, HNH domain and RuvC domain. In our previous study, we have successfully identified the pre-catalytic state of the HNH domain. The purpose of this study is to identify the pre-catalytic state of the RuvC domain, which may provide an understanding of the Cas9 catalytic mechanism and strategies for Cas9 engineering. Methods: Here we use molecular dynamic simulations to discover the pre-catalytic state of Cas9. The initial structure was obtained from our previous simulations, where the HNH domain was in the pre-catalytic state. We placed two Mg2+ ions and non-target DNA strand at the RuvC domain and performed molecular dynamics simulations to capture the pre-catalytic state of the RuvC domain. Results: Our molecular dynamics simulations revealed a pre-catalytic state of Cas9 that both target DNA strand and non-target DNA strand are posed to be catalyzed by Cas9. Conclusions: In this study, we identified the first atomic-level structure of CRISPR/Cas9 with the pre-catalytic state in both catalytic domains. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12503/30338 | |
| dc.language.iso | en | |
| dc.title | Characterize the Pre-catalytic State of CRISPR/Cas9 | |
| dc.type | poster | |
| dc.type.material | text |