Sex and strain differences in renal hemodynamics in mice

dc.creatorTao, Yu
dc.creatorYoung-Stubbs, Cassandra M.
dc.creatorYazdizadeh Shotorbani, Parisa
dc.creatorSu, Dong-Ming
dc.creatorMathis, Keisa W.
dc.creatorMa, Rong
dc.creator.orcid0000-0002-5896-9178 (Ma, Rong)
dc.creator.orcid0000-0002-3326-4753 (Su, Dong-Ming)
dc.description.abstractThe present study was to examine sex and strain differences in glomerular filtration rate (GFR) and renal blood flow (RBF) in C57BL6, 129/Sv, and C57BLKS/J mice, three commonly used mouse strains in renal research. GFR was measured by transdermal measurement of FITC-sinitrin clearance in conscious mice. RBF was measured by a flow probe placed in the renal artery under an anesthetic state. In C57BL6 mice, there were no sex differences in both GFR and RBF. In 129/Sv mice, females had significantly greater GFR than males at age of 24 weeks, but not at 8 weeks. However, males had higher RBF and lower renal vascular resistance (RVR). Similar to 129/Sv, female C57BLKS/J had significantly greater GFR at both 8 and 24 weeks, lower RBF, and higher RVR than males. Across strains, male 129/Sv had lower GFR and higher RBF than male C57BL6, but no significant difference in GFR and greater RBF than male C57BLKS/J. No significant difference in GFR or RBF was observed between C57BL6 and C57BLKS/J mice. Deletion of eNOS in C57BLKS/J mice reduced GFR in both sexes, but decreased RBF in males. Furthermore, there were no sex differences in the severity of renal injury in eNOS(-/-) dbdb mice. Taken together, our study suggests that sex differences in renal hemodynamics in mice are strain and age dependent. eNOS was not involved in the sex differences in GFR, but in RBF. Furthermore, the sexual dimorphism did not impact the severity of renal injury in diabetic nephropathy.
dc.description.sponsorshipThe work was supported by National Institutes of Health Grant R01s (NIH/NIDDK, DK115424- 01 to R. Ma; NIH/NIHL, 1R01HL153703- 01A1 to KW. Mathis), the Translational Project Award from American Heart Association (20TPA35500045, to R. Ma), The Ideal Award from Department of Defense (LR210096 to KW. Mathis), and American Heart Association Predoctoral Fellowship (22PRE903925, to Y. Tao).
dc.identifier.citationTao, Y., Young-Stubbs, C., Yazdizadeh Shotorbani, P., Su, D. M., Mathis, K. W., & Ma, R. (2023). Sex and strain differences in renal hemodynamics in mice. Physiological reports, 11(6), e15644.
dc.publisherWiley Periodicals, Inc.
dc.rights.holder© 2023 The Authors.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.sourcePhysiological Reports
dc.subjectglomerular filtration rate
dc.subjectrenal blood flow
dc.subjectrenal hemodynamics
dc.subject.meshMice, Inbred C57BL
dc.subject.meshKidney / blood supply
dc.subject.meshHemodynamics / physiology
dc.subject.meshRenal Circulation / physiology
dc.subject.meshVascular Resistance
dc.subject.meshGlomerular Filtration Rate / physiology
dc.titleSex and strain differences in renal hemodynamics in mice


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