The Regulation of P53 by Protein Kinase C in Anticancer Drug-Induced Apoptosis

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dc.contributor.advisorBasu, Alakananda
dc.contributor.committeeMemberWu, Ming-Chi
dc.creatorJohnson, Cassie L.
dc.date.accessioned2019-08-22T19:44:18Z
dc.date.available2019-08-22T19:44:18Z
dc.date.issued2001-12-01
dc.date.submitted2014-02-11T06:49:11-08:00
dc.description.abstractJohnson, C., The regulation of p53 by protein kinase C in anticancer drug-induced apoptosis. Master of Science (Microbiology and Immunology), December, 2001. 43 pp., 11 figures, references, 6 titles. The tumor suppression protein p53 has been implicated in DNA damage-induced apoptosis. Previous studies demonstrated that the protein kinase C (PKC) signal transduction pathway regulates apoptosis induced by the DNA damaging agent cisplatin and is deregulated in cisplatin-resistant cells. The present study examined whether PKC influences p53 and, hence, cellular sensitivity to cisplatin. Basal p53 levels were elevated in cisplatin-resistant HeLa (HeLa/CP) cells as compared to parental HeLa cells. Cisplatin further increased p53 levels in HeLA/CP, but not in HeLA cells. However, rottlerin, a PKC-δ inhibitor that prevents cisplatin-induced apoptosis, caused p53 accumulation in HeLa cells treated with cisplatin. Rottlerin stabilized p53 in response to cisplatin in HeLa cells, whereas cisplatin alone was sufficient to stabilize p53 in HeLa/CP cells.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/26869
dc.language.isoen
dc.provenance.legacyDownloads1
dc.subjectCancer Biology
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectCellular and Molecular Physiology
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectBasal P53
dc.subjectprotein kinase C
dc.subjectanticancer-drug induced apoptosis
dc.subjecttumor suppression protein
dc.subjectDNA damage
dc.subjectrottlerin
dc.subjectPKC-δ
dc.subjectPKC
dc.subjectHeLA cells
dc.titleThe Regulation of P53 by Protein Kinase C in Anticancer Drug-Induced Apoptosis
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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