Production of Extracellular Matrix-Degrading Proteases by a Rat B Cell Line.CRL-1631

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dc.contributor.advisorR. Goldfarb
dc.creatorBadeaux, Kathleen S.
dc.date.accessioned2019-08-22T21:43:32Z
dc.date.available2019-08-22T21:43:32Z
dc.date.issued2002-05-01
dc.date.submitted2013-11-22T14:48:04-08:00
dc.description.abstractBadeaux, K. Production of Extracellular Matrix-Degrading Proteases by a Rat B Cell Line.CRL-1631. Master of Science (Microbiology and Immunology), May 2002. 30 pp., 9 illustrations, 1 table, 16 bibliography titles. Previously B lymphocytes have been reported to accumulate at the site of tumor development and to play a role in immune surveillance against metastatic tumors. Investigating this mechanism, we studied B lymphocyte production of extracellular matrix-degrading proteinases: matrix metalloproteinases (MMPs) and components of the urokinase plasminogen activator (uP A) system. Our studies include RT-PCR of CRL-1631 eDNA revealing mRNA for MMP-2, MMP-9, TIMP-1, TIMP-2 and uPAR. MMP-2 and MMP-9 activity was verified by gelatin zymography. TIMP-1, TIMP-2 and uPAR protein expression was confirmed by Western blot analyses. I also report, for the first time, MT -1 MMP gene and protein expression in B cells by RT-PCR and Western blot, respectively. CRL-1631 invasion through Matrigel model basement membrane was significantly inhibited by BB-94, confirming MMP involvement in this cell line's invasiveness. Therefore, B cells use multiple proteases in the degradation of the extracellular matrix, ECM, and this may be one factor responsible for their accumulation at the site of established tumors.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29538
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectGenetics and Genomics
dc.subjectMedicine and Health Sciences
dc.subjectMolecular Genetics
dc.subjectOncology
dc.subjectLymphocytes
dc.subjecttumors
dc.subjectcancer
dc.subjectgenetics
dc.subjectprotein expression
dc.titleProduction of Extracellular Matrix-Degrading Proteases by a Rat B Cell Line.CRL-1631
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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