Conformational Properties of Circulating and Recombinant Forms of Human Plasma Lecithin Cholesterol ACYL Transferase

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dc.contributor.advisorWalter McConathy
dc.creatorSundarrajan, Geetha
dc.date.accessioned2019-08-22T21:19:00Z
dc.date.available2019-08-22T21:19:00Z
dc.date.issued1995-06-01
dc.date.submitted2014-04-01T09:12:05-07:00
dc.description.abstractSundarrajan, Geetha, Conformational properties of circulating and recombinant forms of human plasma LCAT Master of Science (Biomedical Sciences), June, 1995, 69pp., 3 tables, 18 figures, 47 references. The relationship between enzymatic activity and conformational properties of the circulating and recombinant forms of human plasma LCAT were examined in the native and denatured states. The two denaturing agents used in this study were guanidine hydrochloride and heat. These studies led to the following conclusions: (1) Although the alpha helical content of desialylated recombinant LCAT (d-LCAT) is comparable to that of the other two forms of the enzyme (p-LCAT and r-LCAT), the desialylation of LCAT is associated with an increase in the beta sheet and a decrease in beta turn content. (2) The presence of sialic acids, in addition, seems to influence the local environments of aromatic amino acid residues. (3) From the denaturation and renaturation studies with guanidine hydrochloride and heat, it appears that the N-glycan structures of p-PCAT and r-LCAT may contribute differentially to the conformational stability of the enzyme. (4) The alpha helical structure of LCAT may not be involved in maintaining the active conformation of the enzyme.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29239
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCardiovascular System
dc.subjectCell Anatomy
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCellular and Molecular Physiology
dc.subjectChemicals and Drugs
dc.subjectHemic and Immune Systems
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectConformational properties
dc.subjectcirculating
dc.subjectrecombinant forms
dc.subjecthuman plasma
dc.subjectLCAT
dc.subjectenzymatic activity
dc.subjectnative state
dc.subjectdenatured state
dc.subjectp-LCA
dc.subjectr-LCAT
dc.subjectaromatic amino acid residues
dc.subjectguanidine hydrochloride
dc.subjectheat
dc.subjectalpha helical structure
dc.subjectenzyme
dc.titleConformational Properties of Circulating and Recombinant Forms of Human Plasma Lecithin Cholesterol ACYL Transferase
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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