Effects of Amicon Ultra-4 Centrifugal Devices on DNA Yield From Bone Samples and Effects of Amplicon Rx Post-PCR Treatment onRelative Florescence Units Obtained During Capillary Electrophoresis
dc.contributor.advisor | Arthur Eisenberg | |
dc.creator | Amiel, Marie B. | |
dc.date.accessioned | 2019-08-22T21:21:55Z | |
dc.date.available | 2019-08-22T21:21:55Z | |
dc.date.issued | 2014-05-01 | |
dc.date.submitted | 2014-05-22T08:00:05-07:00 | |
dc.description.abstract | The ability to recover DNA and perform DNA analysis from skeletal remains is a valuable tool for identification of missing persons and unidentified remains. DNA extraction from bone often yields low levels of DNA, which can inhibit analysis and impact genetic profiles. Efficient recovery of DNA from bones is therefore vital. In this study, three molecular weight cut-off columns were evaluated. The AmiconĀ® 10,000 NMWL filter device proved to be most efficient at retaining amplifiable DNA and obtaining optimal genetic analysis results. Additionally, the ability of Amplicon RxTM, a post-PCR treatment, to improve genetic profiles by providing a boost in RFUs obtained during capillary electrophoresis was evaluated. | |
dc.format.mimetype | application/pdf | |
dc.identifier.uri | https://hdl.handle.net/20.500.12503/29276 | |
dc.language.iso | en | |
dc.provenance.legacyDownloads | 644 | |
dc.subject | Genetics | |
dc.subject | Genetics and Genomics | |
dc.subject | Medical Genetics | |
dc.subject | Forensics | |
dc.subject | Bone | |
dc.subject | Amplicon RX | |
dc.subject | Amicon | |
dc.subject | organic extraction | |
dc.subject | Forensic Genetics | |
dc.title | Effects of Amicon Ultra-4 Centrifugal Devices on DNA Yield From Bone Samples and Effects of Amplicon Rx Post-PCR Treatment onRelative Florescence Units Obtained During Capillary Electrophoresis | |
dc.type | Thesis | |
dc.type.material | text | |
thesis.degree.discipline | Biomedical Sciences | |
thesis.degree.grantor | University of North Texas Health Science Center at Fort Worth | |
thesis.degree.name | Master of Science |
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