Effects of Amicon Ultra-4 Centrifugal Devices on DNA Yield From Bone Samples and Effects of Amplicon Rx Post-PCR Treatment onRelative Florescence Units Obtained During Capillary Electrophoresis

dc.contributor.advisorArthur Eisenberg
dc.creatorAmiel, Marie B.
dc.date.accessioned2019-08-22T21:21:55Z
dc.date.available2019-08-22T21:21:55Z
dc.date.issued2014-05-01
dc.date.submitted2014-05-22T08:00:05-07:00
dc.description.abstractThe ability to recover DNA and perform DNA analysis from skeletal remains is a valuable tool for identification of missing persons and unidentified remains. DNA extraction from bone often yields low levels of DNA, which can inhibit analysis and impact genetic profiles. Efficient recovery of DNA from bones is therefore vital. In this study, three molecular weight cut-off columns were evaluated. The Amicon® 10,000 NMWL filter device proved to be most efficient at retaining amplifiable DNA and obtaining optimal genetic analysis results. Additionally, the ability of Amplicon RxTM, a post-PCR treatment, to improve genetic profiles by providing a boost in RFUs obtained during capillary electrophoresis was evaluated.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29276
dc.language.isoen
dc.provenance.legacyDownloads644
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectMedical Genetics
dc.subjectForensics
dc.subjectBone
dc.subjectAmplicon RX
dc.subjectAmicon
dc.subjectorganic extraction
dc.subjectForensic Genetics
dc.titleEffects of Amicon Ultra-4 Centrifugal Devices on DNA Yield From Bone Samples and Effects of Amplicon Rx Post-PCR Treatment onRelative Florescence Units Obtained During Capillary Electrophoresis
dc.typeThesis
dc.type.materialtext
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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