CD44 EXPRESSION INDUCES CALCIUM INFLUX DECREASING EGR-1 EXPRESSION AND PROLIFERATION IN ACUTE LYMPHOCYTIC LEUKEMIA CELLS

Simple item page
dc.creatorRacine, Ronny R.
dc.creatorMummert, Mark E.
dc.date.accessioned2019-08-22T19:50:40Z
dc.date.available2019-08-22T19:50:40Z
dc.date.issued2014-03
dc.date.submitted2014-01-29T17:53:13-08:00
dc.descriptionResearch Appreciation Day Award Winner - 2014 Graduate School of Biomedical Sciences Awards, Department of Cell Biology - 1st Place Poster Award
dc.descriptionResearch Appreciation Day Award Winner - 2014 Graduate Student Association Awards - 3rd Place Poster
dc.description.abstractCD44 is a cell surface glycoprotein that serves as the major receptor for hyaluronan, aiding in trafficking and adhesion of immune cells. CD44 also serves as a recruitment platform for signaling molecules and has been shown to regulate proliferation. We have shown that CD44 expression in Jurkat T cells causes a decrease in proliferation. In our current study, we have observed that CD44 expression greatly increases the influx of calcium from extracellular sources. Calcium influx is necessary for the proliferation of T cells, but CD44 expressing Jurkat cells show a disrupted calcium homeostasis. Through use of calcium channel inhibitors we have shown that Jurkat T cells rely on calcium release activated calcium channels for influx. We have observed that CD44 induced excess calcium influx negatively regulates early growth response protein 1 expression, which is responsible for the decrease in proliferation. Our findings show for the first time that CD44 can influence the calcium signaling of leukemic T cells, impacting their proliferation and potentially making a less aggressive cancer cell. Purpose (a): CD44 is a cell surface glycoprotein that serves as the major receptor for hyaluronan, aiding in trafficking and adhesion of immune cells. CD44 also serves as a recruitment platform for signaling molecules and has been shown to regulate proliferation. We have shown that CD44 expression in Jurkat T cells causes a decrease in proliferation. Methods (b): In our current study, we have observed that CD44 expression greatly increases the influx of calcium from extracellular sources. Calcium influx is necessary for the proliferation of T cells, but CD44 expressing Jurkat cells show a disrupted calcium homeostasis. Through use of calcium channel inhibitors we have shown that Jurkat T cells rely on calcium release activated calcium channels for influx. Results (c): We have observed that CD44 induced excess calcium influx negatively regulates early growth response protein 1 expression, which is responsible for the decrease in proliferation. Conclusions (d): Our findings show for the first time that CD44 can influence the calcium signaling of leukemic T cells, impacting their proliferation and potentially making a less aggressive cancer cell.
dc.identifier.urihttps://hdl.handle.net/20.500.12503/27090
dc.language.isoen
dc.subjectleukemia
dc.subjectCD44
dc.subjectcalcium influx
dc.titleCD44 EXPRESSION INDUCES CALCIUM INFLUX DECREASING EGR-1 EXPRESSION AND PROLIFERATION IN ACUTE LYMPHOCYTIC LEUKEMIA CELLS
dc.typeposter
dc.type.materialtext

Files

Collections

Immunology