Involvement of Caspase-2 in Cisplatin-Induced Cell Death in 2008 Ovarian Cancer Cells

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dc.contributor.advisorBasu, Alakananda
dc.contributor.committeeMemberBerg, Rance E.
dc.contributor.committeeMemberGryczynski, Zygmunt
dc.creatorAdkins, Brett T.
dc.date.accessioned2019-08-22T19:35:38Z
dc.date.available2019-08-22T19:35:38Z
dc.date.issued2008-04-01
dc.date.submitted2013-11-18T12:54:15-08:00
dc.description.abstractAdkins, B., Involvement of caspase-2 in cisplatin-induced cell death in 2008 ovarian cancer cells. Master of Science (Molecular Biology and Immunology) April, 2008, 59 pp., 12 illustrations, bibliography, 73 titles. Cisplatin, one of the most effective anticancer drugs in the treatment of ovarian cancer, causes DNA damage and leads to apoptosis. Caspases, a family of cysteine proteases, are essential for the induction of apoptosis. Initiator caspases activate effector caspases to trigger apoptosis. Caspase-2 can function as both an initiator and effector caspase although there are controversies regarding its role in DNA damage-induced apoptosis. Caspase-2 is the only caspase constitutively located in the nucleus, although its function there is unknown. In the present study we have investigated if caspase-2 is important during cisplatin-induced apoptosis and whether cisplatin treatment affects the localization of caspase-2. Caspase-2 depletion suggested that caspase-2 acts upstream of caspase-2 acts upstream of caspase-9 in cisplatin-induced apoptosis. We also made a novel observation that rottlerin, an inhibitor of DNA damage-induced apoptosis, specifically downregulates caspase-2 via the ubiquitin proteamose-mediated pathway. We further show that cisplatin induces caspase-2 translocation out of the nucleus. Moreover, translocation of caspase-2 is more important for cisplatin-induced cell death.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/26347
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCancer Biology
dc.subjectCell Anatomy
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectCellular and Molecular Physiology
dc.subjectDevelopmental Biology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenomics
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectMicrobiology
dc.subjectMolecular Genetics
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectCell death
dc.subjectovarian cancer cells
dc.subjectcisplatin-induced cell death
dc.subjectcapase-2
dc.subjectcysteine proteases
dc.subjectapoptosis
dc.subjectDNA damage
dc.subjectrottlerin
dc.subjecttranslocation
dc.titleInvolvement of Caspase-2 in Cisplatin-Induced Cell Death in 2008 Ovarian Cancer Cells
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineEpidemiology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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