Validation and Optimization of Automated Instrumentation for Mitochondrial DNA Database Sample Processing

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dc.contributor.advisorJohn Planz
dc.contributor.committeeMemberArthur Eisenberg
dc.contributor.committeeMemberJoseph Warren
dc.creatorCrispin, Shelley Jeanine
dc.date.accessioned2019-08-22T19:36:46Z
dc.date.available2019-08-22T19:36:46Z
dc.date.issued2004-06-01
dc.date.submitted2014-05-05T12:18:05-07:00
dc.description.abstractCrispin, Shelley Jeanine. Validation and Optimization of Automated Instrumentation for Mitochondrial DNA Database Sample Processing. Master of Science (Forensic Genetics), August 2004, 43 pages, 8 figures, 8 tables, 26 references. The validation of Qiagen 9604 BioRobot for DNA extraction and two Corbett CAS-1200 Liquid Precision Handling Systems for mitochondrial DNA database sample processing will alleviate sample backlogs and increase sample throughput. A manual cross-contamination study and an automated cross-contamination study showed that the use of automated instrumentation allowed for quicker and more accurate sample processing. The validation process was completed by processing a 96-well high throughput plate containing AFDIL employee samples. AFDIL loses approximately 10% of samples cycle sequenced using quarter volume BigDye terminator cycle reactions. To validate the use of half volume BigDye terminator cycled sequencing a 96-well high throughput plate containing AFDIL employee samples was used to compare the percentage of samples lost. On average 1.6% of the samples were lost per 96-well high throughput plate utilizing half volume BigDye terminator cycle sequencing reactions. This study has shown that the validation of half volume BigDye terminator cycle sequencing reactions will minimize the number of samples that will have to be reprocessed per 96-well high throughput plate.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/26425
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectComputational Biology
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Genetics and Genomics
dc.subjectValidation
dc.subjectoptimization
dc.subjectautomated instrumentation
dc.subjectmitochondrial DNA database sample processing
dc.subjectQiagen 9604 BioRobot
dc.subjectDNA extraction
dc.subjectCorbett CAS-1200 Liquid Precision Handling Systems
dc.subjectsample backlogs
dc.subjectcross-contamination study
dc.subjecthigh throughput plate
dc.subjectvalidation
dc.subjectBigDye terminator cycle
dc.titleValidation and Optimization of Automated Instrumentation for Mitochondrial DNA Database Sample Processing
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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