Browsing by Subject "trace DNA"
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Item Alternative procedure for DNA recovery from cartridges and casings(2020-05) Bettex, Kelsey D.; Planz, John V.; Jones, Harlan P.; Warren, Joseph E.Both unfired and fired ammunition recovered from a crime scene are useful in connecting a crime to a specific firearm, however its use in identifying individuals is less common due to low-level or degraded DNA present. Traditionally, the standard procedure for DNA sampling of ammunition evidence is the use of a swabbing technique. The New York State Police (NYSP) Forensic Investigation Center conducted a study to test the efficacy of an alternative method for obtaining DNA from cartridges and casings, soaking the sample prior to extraction. This method presented a 186% increase in total DNA yield, 169% increase in male DNA yield, and a 44% increase in comparable profiles obtained using GlobalFiler(TM) and 240% using Yfiler(R) Plus. Results showed fewer 'no data' determinations using the soaking method, indicating more DNA obtained. Although a statistically significant difference was not observed between the two methods (p = 0.070), a qualitative significance was.Item Analysis of HemaSpot[TM]-HF and HemaSpot[TM]-HD Sampling Kits Using Trace DNA(2019-05) McGehee, Rachel L.; Warren, Joseph E.; Planz, John V.; Schiro, GeorgeSpot On Sciences, Inc. has recently developed a new device, the HemaSpot[TM], which allows for samples to be stored safely at ambient temperatures. The UNTHSC FGEN program was contacted to conduct a research study to determine its feasibility for use as collection and storage media with trace samples. Extractions of 108 samples were conducted with QIAGEN[R] QIAamp DNA Investigator Kits, a 3130xL Genetic Analyzer, and GeneMapper[R] ID-X software. A hypersensitivity study worked with sub-optimal amounts of control DNA in order to observe the quality and variation of the generated profiles. The trace study swabbed items found at typical crime scenes and determined the device's ability to generate readable profiles. Results uncovered that all samples either contained large portions of allelic dropout or contamination. Relatively similar partial profiles were produced for both cartridge types in the hypersensitivity study. In addition, readable trace profiles were compared to one another to conclude that the HemaSpot[TM]-HD had the most success, however this may have been the cause of limited size and sample variation. Both products should be tested further.