Results of a collaborative study on DNA identification of aged bone samples

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dc.creatorVanek, Daniel
dc.creatorBudowle, Bruce
dc.creatorDubska-Votrubova, Jitka
dc.creatorAmbers, Angie
dc.creatorFrolik, Jan
dc.creatorPospisek, Martin
dc.creatorAl Afeefi, Ahmed Anwar
dc.creatorAl Hosani, Khalid Ismaeil
dc.creatorAllen, Marie
dc.creatorAl Naimi, Khudooma Saeed
dc.creatorAl Salafi, Dina
dc.creatorAl Tayyari, Wafa Ali Rashid
dc.creatorArgueta, Wendy C.
dc.creatorBottinelli, Michel
dc.creatorBus, Magdalena M.
dc.creatorCemper-Kiesslich, Jan
dc.creatorCepil, Olivier
dc.creatorDe Cock, Greet
dc.creatorDesmyter, Stijn
dc.creatorEl Amri, Hamid
dc.creatorEl Ossmani, Hicham
dc.creatorGaldies, Ruth
dc.creatorGrun, Sebastian
dc.creatorGuidet, Francois
dc.creatorHoefges, Anna
dc.creatorIancu, Cristian Bogdan
dc.creatorLotz, Petra
dc.creatorMaresca, Alessandro
dc.creatorNagy, Marion
dc.creatorNovotny, Jindrich
dc.creatorRachid, Hajar
dc.creatorRothe, Jessica
dc.creatorStenersen, Marguerethe
dc.creatorStephenson, Mishel
dc.creatorStevanovitch, Alain
dc.creatorStrien, Juliane
dc.creatorSumita, Denilce R.
dc.creatorVella, Joanna
dc.creatorZander, Judith
dc.creator.orcid0000-0002-6733-8300 (Bus, Magdalena M.)
dc.date.accessioned2022-08-22T20:39:14Z
dc.date.available2022-08-22T20:39:14Z
dc.date.issued2017-06-14
dc.description.abstractAIM: A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. METHODS: Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. RESULTS: Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. CONCLUSION: The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.
dc.description.sponsorshipThe work on this project was partly supported by the Czech Science Foundation, grant No. 14-36938G.
dc.identifier.citationVanek, D., Budowle, B., Dubska-Votrubova, J., Ambers, A., Frolik, J., Pospisek, M., Al Afeefi, A. A., Al Hosani, K. I., Allen, M., Al Naimi, K. S., Al Salafi, D., Al Tayyari, W., Arguetaa, W., Bottinelli, M., Bus, M. M., Cemper-Kiesslich, J., Cepil, O., De Cock, G., Desmyter, S., El Amri, H., ... Zander, J. (2017). Results of a collaborative study on DNA identification of aged bone samples. Croatian medical journal, 58(3), 203-213. https://doi.org/10.3325/cmj.2017.58.203
dc.identifier.issn1332-8166
dc.identifier.issue3
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31610
dc.identifier.volume58
dc.publisherMedicinska naklada
dc.relation.urihttps://doi.org/10.3325/cmj.2017.58.203
dc.rights.holder© 2017 by the Croatian Medical Journal.
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceCroatian Medical Journal
dc.subject.meshBone and Bones / chemistry
dc.subject.meshCzech Republic
dc.subject.meshDNA / analysis
dc.subject.meshDNA Fingerprinting / standards
dc.subject.meshForensic Genetics
dc.subject.meshHumans
dc.titleResults of a collaborative study on DNA identification of aged bone samples
dc.typeArticle
dc.type.materialtext

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