Novel Correlation between TGF-beta1/-beta3 and Hormone Receptors in the Human Corneal Stroma

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dc.creatorChoi, Alexander J.
dc.creatorHefley, Brenna S.
dc.creatorNicholas, Sarah E.
dc.creatorCunningham, Rebecca L.
dc.creatorKaramichos, Dimitrios
dc.creator.orcid0000-0002-8761-3824 (Karamichos, Dimitrios)
dc.creator.orcid0000-0001-5984-5516 (Cunningham, Rebecca)
dc.date.accessioned2023-09-14T14:18:39Z
dc.date.available2023-09-14T14:18:39Z
dc.date.issued2023-09-09
dc.description.abstractThis study investigated the interplay between transforming growth factor beta (TGF-beta1/T1 and TGF-beta3/T3), and sex hormone receptors using our 3D in vitro cornea stroma model. Primary human corneal fibroblasts (HCFs) from healthy donors were plated in transwells at 10(6) cells/well and cultured for four weeks. HCFs were supplemented with stable vitamin C (VitC) and stimulated with T1 or T3. 3D construct proteins were analyzed for the androgen receptor (AR), progesterone receptor (PR), estrogen receptor alpha (ERalpha) and beta (ERbeta), luteinizing hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), gonadotropin-releasing hormone receptor (GnRHR), KiSS1-derived peptide receptor (KiSS1R/GPR54), and follicle-stimulating hormone subunit beta (FSH-B). In female constructs, T1 significantly upregulated AR, PR, ERalpha, FSHR, GnRHR, and KiSS1R. In male constructs, T1 significantly downregulated FSHR and FSH-B and significantly upregulated ERalpha, ERbeta, and GnRHR. T3 caused significant upregulation in expressions PR, ERalpha, ERbeta, LHR, FSHR, and GNRHR in female constructs, and significant downregulation of AR, ERalpha, and FSHR in male constructs. Semi-quantitative Western blot findings present the interplay between sex hormone receptors and TGF-beta isoforms in the corneal stroma, which is influenced by sex as a biological variable (SABV). Additional studies are warranted to fully delineate their interactions and signaling mechanisms.
dc.description.sponsorshipThe authors would like to acknowledge the following for their financial support: National Eye Institute, National Institutes of Health; (EY028888).
dc.identifier.citationChoi, A. J., Hefley, B. S., Nicholas, S. E., Cunningham, R. L., & Karamichos, D. (2023). Novel Correlation between TGF-β1/-β3 and Hormone Receptors in the Human Corneal Stroma. International journal of molecular sciences, 24(17), 13635. https://doi.org/10.3390/ijms241713635
dc.identifier.issn1422-0067
dc.identifier.issue17
dc.identifier.urihttps://hdl.handle.net/20.500.12503/32410
dc.identifier.volume24
dc.publisherMDPI
dc.relation.urihttps://doi.org/10.3390/ijms241713635
dc.rights.holder© 2023 by the authors.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceInternational Journal of Molecular Sciences
dc.subject3D in vitro model
dc.subjectSabv
dc.subjectTGF-beta1
dc.subjectTGF-beta3
dc.subjectcornea
dc.subjectcorneal fibrosis
dc.subjectcorneal stroma
dc.subjectsex hormone receptors
dc.subject.meshHumans
dc.subject.meshFemale
dc.subject.meshMale
dc.subject.meshCorneal Stroma
dc.subject.meshTransforming Growth Factor beta3
dc.subject.meshEstrogen Receptor alpha
dc.subject.meshReceptors, Kisspeptin-1
dc.subject.meshEstrogen Receptor beta / genetics
dc.subject.meshTransforming Growth Factor beta1 / genetics
dc.subject.meshTransforming Growth Factor beta
dc.subject.meshFollicle
dc.subject.meshStimulating Hormone
dc.titleNovel Correlation between TGF-beta1/-beta3 and Hormone Receptors in the Human Corneal Stroma
dc.typeArticle
dc.type.materialtext

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