3D Spheroids Derived from Human Lipedema ASCs Demonstrated Similar Adipogenic Differentiation Potential and ECM Remodeling to Non-Lipedema ASCs In Vitro

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dc.creatorAl-Ghadban, Sara
dc.creatorPursell, India A.
dc.creatorDiaz, Zaidmara T.
dc.creatorHerbst, Karen L.
dc.creatorBunnell, Bruce A.
dc.creator.orcid0000-0001-6196-3722 (Bunnell, Bruce A.)
dc.date.accessioned2022-08-25T15:39:43Z
dc.date.available2022-08-25T15:39:43Z
dc.date.issued2020-11-07
dc.description.abstractThe growth and differentiation of adipose tissue-derived stem cells (ASCs) is stimulated and regulated by the adipose tissue (AT) microenvironment. In lipedema, both inflammation and hypoxia influence the expansion and differentiation of ASCs, resulting in hypertrophic adipocytes and deposition of collagen, a primary component of the extracellular matrix (ECM). The goal of this study was to characterize the adipogenic differentiation potential and assess the levels of expression of ECM-remodeling markers in 3D spheroids derived from ASCs isolated from both lipedema and healthy individuals. The data showed an increase in the expression of the adipogenic genes (ADIPOQ, LPL, PPAR-γ and Glut4), a decrease in matrix metalloproteinases (MMP2, 9 and 11), with no significant changes in the expression of ECM markers (collagen and fibronectin), or integrin A5 in 3D differentiated lipedema spheroids as compared to healthy spheroids. In addition, no statistically significant changes in the levels of expression of inflammatory genes were detected in any of the samples. However, immunofluorescence staining showed a decrease in fibronectin and increase in laminin and Collagen VI expression in the 3D differentiated spheroids in both groups. The use of 3D ASC spheroids provide a functional model to study the cellular and molecular characteristics of lipedema AT.
dc.description.sponsorshipThis study was supported by the Lipedema foundation. The grant number: 000009.
dc.identifier.citationAl-Ghadban, S., Pursell, I. A., Diaz, Z. T., Herbst, K. L., & Bunnell, B. A. (2020). 3D Spheroids Derived from Human Lipedema ASCs Demonstrated Similar Adipogenic Differentiation Potential and ECM Remodeling to Non-Lipedema ASCs In Vitro. International journal of molecular sciences, 21(21), 8350. https://doi.org/10.3390/ijms21218350
dc.identifier.issn1422-0067
dc.identifier.issue21
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31640
dc.identifier.volume21
dc.publisherMDPI
dc.relation.urihttps://doi.org/10.3390/ijms21218350
dc.rights.holder© 2020 by the authors.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceInternational Journal of Molecular Sciences
dc.subjectECM markers
dc.subjectadipogenic differentiation
dc.subjectadipose tissue-derived stem cells (ASCs)
dc.subjectinflammation
dc.subjectlipedema
dc.subjectspheroids
dc.subject.meshAdipocytes / metabolism
dc.subject.meshAdipogenesis
dc.subject.meshAdipose Tissue / cytology
dc.subject.meshAdipose Tissue / metabolism
dc.subject.meshCell Culture Techniques / methods
dc.subject.meshCell Differentiation
dc.subject.meshCell Proliferation
dc.subject.meshCells, Cultured
dc.subject.meshExtracellular Matrix / metabolism
dc.subject.meshExtracellular Matrix / physiology
dc.subject.meshHumans
dc.subject.meshLipedema / metabolism
dc.subject.meshMesenchymal Stem Cells / metabolism
dc.subject.meshOrganoids / metabolism
dc.subject.meshStem Cells / metabolism
dc.subject.meshTissue Engineering / methods
dc.title3D Spheroids Derived from Human Lipedema ASCs Demonstrated Similar Adipogenic Differentiation Potential and ECM Remodeling to Non-Lipedema ASCs In Vitro
dc.typeArticle
dc.type.materialtext

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