Metformin Nanoparticles for Liver Delivery




Dong, Xiaowei
Olowookere, Yetunde
Xu, Yong
Yang, Shaohua


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Metformin Nanoparticles for Liver Delivery Purpose: Metformin hydrochloride is a biguanide, an antidiabetic medicine, taken orally to lower blood sugar in diabetic patients, especially with Type 2 diabetes. Metformin is hydrophilic in nature and it has been observed that overtime, higher doses are usually required for effectiveness. This might result from low bioavailability due to its formulation. The purpose of this study was to develop a HPLC- method for Metformin Hydrochloride that will be used for further formulation development. Method: Metformin hydrochloride solution was analyzed quantitatively using Waters HPLC separation module 2695 equipped with auto injector and detector (PDA 2996) and UV detector (2487), Embedded with Empower software. Method was developed by utilizing a reverse phase chromatographic with Column Ultimate XB-SCX Dim 4.6 x250mm, with an ambient temperature, 5µm particle size, Flow rate 1ml/min, Wavelength 232nm, Ammonium dihydrogen phosphate as the mobile phase and injection volume of 10µl. Forced degradation studies were conducted by employing stress conditions with various concentrations of HCl, NaOH, H2O2 for acid hydrolysis, base hydrolysis and oxidation. Results:The detection of the eluent was consistently observed at around 11 minutes for all concentrations. SD value was low, RSD was around 1% indicating accuracy and reproducibility with regression coefficient R ≥ 0.997 with six different concentrations from 1mg/ml-100mg/ml. Conclusion:The quantitative analysis result for the HPLC method was close to 100% and it did not appear there was any interference from the excipients. The method was simple, rapid and reproducible and useful for further drug formulation development.