Common Lung Microbiome Identified among Mechanically Ventilated Surgical Patients

Date

2016-11-29

Authors

Smith, Ashley D.
Zhang, Yan
Barber, Robert C.
Minshall, Christian T.
Huebinger, Ryan M.
Allen, Michael S.

ORCID

0000-0003-2293-8078 (Allen, Michael S.)
0000-0001-5784-4659 (Zhang, Yan)
0000-0001-6857-0286 (Barber, Robert C.)

Journal Title

Journal ISSN

Volume Title

Publisher

PLOS

Abstract

The examination of the pulmonary microbiome in patients with non-chronic disease states has not been extensively examined. Traditional culture based screening methods are often unable to identify bacteria from bronchoalveolar lavage samples. The advancement of next-generation sequencing technologies allows for a culture-independent molecular based analysis to determine the microbial composition in the lung of this patient population. For this study, the Ion Torrent PGM system was used to assess the microbial complexity of culture negative bronchoalveolar lavage samples. A group of samples were identified that all displayed high diversity and similar relative abundance of bacteria. This group consisted of Hydrogenophaga, unclassified Bacteroidetes, Pedobacter, Thauera, and Acinetobacter. These bacteria may be representative of a common non-pathogenic pulmonary microbiome associated within this population of patients.

Description

Citation

Smith, A. D., Zhang, Y., Barber, R. C., Minshall, C. T., Huebinger, R. M., & Allen, M. S. (2016). Common Lung Microbiome Identified among Mechanically Ventilated Surgical Patients. PloS one, 11(11), e0166313. https://doi.org/10.1371/journal.pone.0166313

Rights

©2016 Smith et al.

License

Attribution 4.0 International (CC BY 4.0)