Adipose-Derived Stem Cells from Obese Donors Polarize Macrophages and Microglia toward a Pro-Inflammatory Phenotype

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dc.creatorHarrison, Mark A. A.
dc.creatorWise, Rachel M.
dc.creatorBenjamin, Brooke P.
dc.creatorHochreiner, Emily M.
dc.creatorMohiuddin, Omair A.
dc.creatorBunnell, Bruce A.
dc.creator.orcid0000-0001-6196-3722 (Bunnell, Bruce A.)
dc.date.accessioned2022-08-25T15:39:45Z
dc.date.available2022-08-25T15:39:45Z
dc.date.issued2020-12-25
dc.description.abstractMacrophages and microglia represent the primary phagocytes and first line of defense in the peripheral and central immune systems. They activate and polarize into a spectrum of pro- and anti-inflammatory phenotypes in response to various stimuli. This activation is tightly regulated to balance the appropriate immune response with tissue repair and homeostasis. Disruption of this balance results in inflammatory disease states and tissue damage. Adipose stem cells (ASCs) have great therapeutic potential because of the potent immunomodulatory capabilities which induce the polarization of microglia and macrophages to the anti-inflammatory, M2, phenotype. In this study, we examined the effects of donor heterogeneity on ASC function. Specifically, we investigated the impact of donor obesity on ASC stemness and immunomodulatory abilities. Our findings revealed that ASCs from obese donors (ObASCs) exhibited reduced stem cell characteristics when compared to ASCs from lean donors (LnASCs). We also found that ObASCs promote a pro-inflammatory phenotype in murine macrophage and microglial cells, as indicated by the upregulated expression of pro-inflammatory genes, increased nitric oxide pathway activity, and impaired phagocytosis and migration. These findings highlight the importance of considering individual donor characteristics such as obesity when selecting donors and cells for use in ASC therapeutic applications and regenerative medicine.
dc.description.sponsorshipThis study was financially supported by private funds from Tulane University and Louisiana Board of Regents predoctoral fellowship LEQSF (2017-22)-GF-11 to M.A.A.H.
dc.identifier.citationHarrison, M., Wise, R. M., Benjamin, B. P., Hochreiner, E. M., Mohiuddin, O. A., & Bunnell, B. A. (2020). Adipose-Derived Stem Cells from Obese Donors Polarize Macrophages and Microglia toward a Pro-Inflammatory Phenotype. Cells, 10(1), 26. https://doi.org/10.3390/cells10010026
dc.identifier.issn2073-4409
dc.identifier.issue1
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31643
dc.identifier.volume10
dc.publisherMDPI
dc.relation.urihttps://doi.org/10.3390/cells10010026
dc.rights.holder© 2020 by the authors.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceCells
dc.subjectadipose stem cells (ASCs)
dc.subjectadipose tissue
dc.subjectimmunomodulation
dc.subjectinflammation
dc.subjectmacrophage
dc.subjectmicroglia
dc.subjectobesity
dc.subjectpolarization
dc.subject.meshAdipose Tissue / cytology
dc.subject.meshAdipose Tissue / immunology
dc.subject.meshAnimals
dc.subject.meshCell Differentiation
dc.subject.meshCell Proliferation
dc.subject.meshCoculture Techniques
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshImmunomodulation
dc.subject.meshMacrophages / cytology
dc.subject.meshMacrophages / metabolism
dc.subject.meshMesenchymal Stem Cells / cytology
dc.subject.meshMesenchymal Stem Cells / immunology
dc.subject.meshMice
dc.subject.meshObesity / metabolism
dc.subject.meshPhenotype
dc.subject.meshPrimary Cell Culture
dc.subject.meshRAW 264.7 Cells
dc.titleAdipose-Derived Stem Cells from Obese Donors Polarize Macrophages and Microglia toward a Pro-Inflammatory Phenotype
dc.typeArticle
dc.type.materialtext

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