The Validation of Applied Biosystems Quantifiler Human DNA Quantification Kit and Quantifiler Y Human Male DNA Quantification Kit for the Armed Forces DNA Identification Laboratory

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dc.contributor.advisorArthur Eisenberg
dc.contributor.committeeMemberJoseph Warren
dc.contributor.committeeMemberJohn Planz
dc.creatorLevandowsky, Elizabeth C.
dc.date.accessioned2019-08-22T21:14:17Z
dc.date.available2019-08-22T21:14:17Z
dc.date.issued2004-08-01
dc.date.submitted2014-05-30T08:00:05-07:00
dc.description.abstractLevandowsky, Elizabeth C., The Validation of Applied Biosystems Quantifiler™ Human DNA Quantification Kit and Quantifier™ Y Human Male DNA Identification Kit for the Armed Forces DNA Identification Laboratory. Master of Science (Forensic Genetics), July 2004. 14 tables, 6 figures, references, 12 titles. To produce the most accurate and reliable results, forensic laboratories, such as AFDIL, must examine new technologies in the field of DNA analysis. The present study is the beginning of an internal validation of the most recent development in DNA quantitation, RT-PCR. The Quantifiler Human DNA Quantification Kit and Quantifiler Y Human Male DNA Identification Kit are RT-PCR assays that quantitate human and male DNA, and detect PCR inhibitors which may hinder the ability to produce a reliable STR profile. Sensitivity and non-probative case sample studies were performed according to the DAB guidelines. The Quantifiler kits were not as sensitive as had been previously reported by Applied Biosystems. The non-probative case sample study demonstrated results two fold greater than results from the Taqman® Alu-PCR Quantitation System, a RT-PCR assay developed and validated at AFDIL. At this time, it appears it may be in the best interest of AFDIL to continue its use of the Taqman® Alu-PCR Quantitation System.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29179
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectComputational Biology
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Genetics and Genomics
dc.subjectValidation
dc.subjectapplied Biosystems quantifiler
dc.subjecthuman DNA quantification kit
dc.subjectquantifiler Y human male DNA identification kit
dc.subjectArmed Forces DNA Identification Laboratory
dc.subjectforensic science
dc.subjectDNA analysis
dc.subjectPCR inhibitors
dc.subjectcase sample
dc.subjectAFDIL
dc.subjectTaqman Alu-PCR Quantitation System
dc.titleThe Validation of Applied Biosystems Quantifiler Human DNA Quantification Kit and Quantifiler Y Human Male DNA Quantification Kit for the Armed Forces DNA Identification Laboratory
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineCell Biology and Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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