Identification of Actin Binding Proteins Associated with Cross-Linked Actin Networks

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dc.contributor.advisorClark, Abbot F.
dc.contributor.committeeMemberYorio, Thomas
dc.contributor.committeeMemberWordinger, Robert J.
dc.creatorMills, Christy E.
dc.date.accessioned2019-08-22T21:29:50Z
dc.date.available2019-08-22T21:29:50Z
dc.date.issued2006-12-01
dc.date.submitted2014-03-18T14:12:05-07:00
dc.description.abstractMills, Christy E., Identification of Actin Binding Proteins Associated with Cross-Lined Actin Networks. Master of Science (Pharmacology and Neuroscience), December 2006, 95 pp., 9 tables, 16 figures, references, 122 titles. Glucocorticoid therapy can leady to ocular hypertension and glaucoma. The purpose of this study is to examine mechanisms contributing to increased intraocular pressure using tissue culture models of steroid-induced ocular hypertension through identification of specific actin-binding proteins associated with cross-linked actin network (CLANs). Human trabecular meshwork ™ cells were cultured to confluence and treated with dexamethasone or vehicle for 14 days. Total RNA was extracted for gene expression analysis to confirm steroid-induced expression of actin binding proteins in human TM cells. Western blots confirmed expression of actin binding proteins and demonstrated the specificity of selected antibodies. Fluorescence microscopy of treated TM cells showed cytoskeleton rearrangements from linear actin stress fibers to cross-linked actin networks and the position of candidate proteins in relation to CLANs. Dexamethasone treatment of TM cells altered the expression of actin-associated proteins that may be important in the formation of CLANs and increased outflow resistance.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29376
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectLife Sciences
dc.subjectMedical Neurobiology
dc.subjectMedicine and Health Sciences
dc.subjectMolecular and Cellular Neuroscience
dc.subjectNervous System
dc.subjectNeuroscience and Neurobiology
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Neuroscience and Neurobiology
dc.subjectIdentification
dc.subjectactin binding proteins
dc.subjectcross-linked actin networks
dc.subjectglucocorticoid therapy
dc.subjectintraocular pressure
dc.subjectocular hypertension
dc.subjectCLANs
dc.subjecttissue model cultures
dc.subjectsteroid-induced
dc.subjectHuman trabecular meshwork cells
dc.subjectRNA
dc.subjectgene expression
dc.subjectdexamethasone
dc.subjectTM cells
dc.titleIdentification of Actin Binding Proteins Associated with Cross-Linked Actin Networks
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplinePharmacology and Neuroscience
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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