Enhanced Angiogenesis in HUVECs Preconditioned with Media from Adipocytes Differentiated from Lipedema Adipose Stem Cells In Vitro

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dc.creatorAl-Ghadban, Sara
dc.creatorWalczak, Samantha G.
dc.creatorIsern, Spencer U.
dc.creatorMartin, Elizabeth C.
dc.creatorHerbst, Karen L.
dc.creatorBunnell, Bruce A.
dc.creator.orcid0000-0001-6196-3722 (Bunnell, Bruce A.)
dc.date.accessioned2023-09-14T14:18:39Z
dc.date.available2023-09-14T14:18:39Z
dc.date.issued2023-09-09
dc.description.abstractLipedema is a connective tissue disorder characterized by increased dilated blood vessels (angiogenesis), inflammation, and fibrosis of the subcutaneous adipose tissue. This project aims to gain insights into the angiogenic processes in lipedema using human umbilical vein endothelial cells (HUVECs) as an in vitro model. HUVECs were cultured in conditioned media (CM) collected from healthy (non-lipedema, AQH) and lipedema adipocytes (AQL). The impacts on the expression levels of multiple endothelial and angiogenic markers [CD31, von Willebrand Factor (vWF), angiopoietin 2 (ANG2), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMPs), NOTCH and its ligands] in HUVECs were investigated. The data demonstrate an increased expression of CD31 and ANG2 at both the gene and protein levels in HUVECs treated with AQL CM in 2D monolayer and 3D cultures compared to untreated cells. Furthermore, the expression of the vWF, NOTCH 4, and DELTA-4 genes decreased. In contrast, increased VEGF, MMP9, and HGF gene expression was detected in HUVECs treated with AQL CM cultured in a 2D monolayer. In addition, the results of a tube formation assay indicate that the number of formed tubes increased in lipedema-treated HUVECs cultured in a 2D monolayer. Together, the data indicate that lipedema adipocyte-CM promotes angiogenesis through paracrine-driven mechanisms.
dc.description.sponsorshipThis work was supported by the Lipedema Foundation (LF 39_21).
dc.identifier.citationAl-Ghadban, S., Walczak, S. G., Isern, S. U., Martin, E. C., Herbst, K. L., & Bunnell, B. A. (2023). Enhanced Angiogenesis in HUVECs Preconditioned with Media from Adipocytes Differentiated from Lipedema Adipose Stem Cells In Vitro. International journal of molecular sciences, 24(17), 13572. https://doi.org/10.3390/ijms241713572
dc.identifier.issn1422-0067
dc.identifier.issue17
dc.identifier.urihttps://hdl.handle.net/20.500.12503/32409
dc.identifier.volume24
dc.publisherMDPI
dc.relation.urihttps://doi.org/10.3390/ijms241713572
dc.rights.holder© 2023 by the authors.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceInternational Journal of Molecular Sciences
dc.subjectangiogenesis
dc.subjectendothelial cells
dc.subjectlipedema
dc.subjectnotch signaling
dc.subject.meshHumans
dc.subject.meshVascular Endothelial Growth Factor A / genetics
dc.subject.meshHuman Umbilical Vein Endothelial Cells
dc.subject.meshvon Willebrand Factor / genetics
dc.subject.meshLipedema
dc.subject.meshAdipocytes
dc.subject.meshCulture Media, Conditioned / pharmacology
dc.subject.meshStem Cells
dc.titleEnhanced Angiogenesis in HUVECs Preconditioned with Media from Adipocytes Differentiated from Lipedema Adipose Stem Cells In Vitro
dc.typeArticle
dc.type.materialtext

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