A Reliable Nonhuman Primate Model of Ischemic Stroke with Reproducible Infarct Size and Long-term Sensorimotor Deficits

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dc.creatorLin, Xiao
dc.creatorWang, Hua
dc.creatorHuang, Shengwei
dc.creatorChen, Lefu
dc.creatorYang, Su
dc.creatorZhao, Peiqi
dc.creatorLin, Zhongxiao
dc.creatorYang, Jianjing
dc.creatorRuan, Linhui
dc.creatorNi, Haoqi
dc.creatorWang, Kankai
dc.creatorWen, Min
dc.creatorJin, Kunlin
dc.creatorZhuge, Qichuan
dc.creator.orcid0000-0002-1336-348X (Jin, Kunlin)
dc.date.accessioned2023-03-27T15:24:23Z
dc.date.available2023-03-27T15:24:23Z
dc.date.issued2023-02-24
dc.description.abstractA nonhuman primate model of ischemic stroke is considered as an ideal preclinical model to replicate various aspects of human stroke because of their similarity to humans in genetics, neuroanatomy, physiology, and immunology. However, it remains challenging to produce a reliable and reproducible stroke model in nonhuman primates due to high mortality and variable outcomes. Here, we developed a focal cerebral ischemic model induced by topical application of 50% ferric chloride (FeCl(3)) onto the MCA-M1 segment through a cranial window in the cynomolgus monkeys. We found that FeCl(3) rapidly produced a stable intraarterial thrombus that caused complete occlusion of the MCA, leading to the quick decrease of the regional cerebral blood flow in 10 min. A typical cortical infarct was detected 24 hours by magnetic resonance imaging (MRI) and was stable at least for 1 month after surgery. The sensorimotor deficit assessed by nonhuman primate stroke scale was observed at 1 day and up to 3 months after ischemic stroke. No spontaneous revascularization or autolysis of thrombus was observed, and vital signs were not affected. All operated cynomolgus monkeys survived. Our data suggested that FeCl(3)-induced stroke in nonhuman primates was a replicable and reliable model that is necessary for the correct prediction of the relevance of experimental therapeutic approaches in human beings.
dc.description.sponsorshipThis research was funded by grants from the National Natural Science Foundation of China (81820108011 and 82001225), Zhejiang provincial natural Science Foundation (2020C03022 and 2017C03027), and Major Science and Technology Project of Wenzhou Science and Technology Bureau (ZS2017007).
dc.identifier.citationLin, X., Wang, H., Huang, S., Chen, L., Yang, S., Zhao, P., Lin, Z., Yang, J., Ruan, L., Ni, H., Wang, K., Wen, M., Jin, K., & Zhuge, Q. (2023). A Reliable Nonhuman Primate Model of Ischemic Stroke with Reproducible Infarct Size and Long-term Sensorimotor Deficits. Aging and disease, 14(1), 245-255. https://doi.org/10.14336/AD.2022.0722
dc.identifier.issn2152-5250
dc.identifier.issue1
dc.identifier.urihttps://hdl.handle.net/20.500.12503/32060
dc.identifier.volume14
dc.publisherAging and Disease
dc.relation.urihttps://doi.org/10.14336/AD.2022.0722
dc.rights.holder© 2022 Lin X. et al.
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceAging and Disease
dc.subjectcynomolgus macaque
dc.subjectferric chloride
dc.subjectischemic stroke
dc.subjectmiddle cerebral artery
dc.subjectnonhuman primate
dc.titleA Reliable Nonhuman Primate Model of Ischemic Stroke with Reproducible Infarct Size and Long-term Sensorimotor Deficits
dc.typeArticle
dc.type.materialtext

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