Competitive Displacement Restores the Hyperpolarized (15)N NMR Signal in Blood Plasma

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dc.creatorSuh, Eul H.
dc.creatorKovacs, Zoltan
dc.creator.orcid0000-0002-3919-678X (Suh, Eul H.)
dc.date.accessioned2023-04-12T20:00:12Z
dc.date.available2023-04-12T20:00:12Z
dc.date.issued2023-03-28
dc.description.abstractHyperpolarized (HP) NMR can improve the sensitivity of conventional NMR experiments by several orders of magnitude, thereby making it feasible to detect the signal of low sensitivity nuclei such as (13)C and (15)N nuclei in vivo. Hyperpolarized substrates are usually administered by direct injection into the bloodstream, and interaction with serum albumin can cause rapid decay of the hyperpolarized signal due to the shortening of the spin-lattice (T(1)) relaxation time. Here we report that the (15)N T(1) of (15)N labeled, partially deuterated tris(2-pyridylmethyl)amine decreases dramatically upon binding to albumin to such an extent that no HP-(15) signal could be detected. We also demonstrate that the signal could be restored using a competitive displacer, iophenoxic acid, which binds stronger to albumin than tris(2-pyridylmethyl)amine. The methodology presented here eliminates the undesirable effect of albumin binding and should widen the range of hyperpolarized probes for in vivo studies.
dc.description.sponsorshipFunding from NIH (P41EB015908, R21EB030765, R21EB031367) is acknowledged. In memory of S. James Ratnakar (1975-2022).
dc.identifier.citationSuh, E. H., & Kovacs, Z. (2022). Competitive Displacement Restores the Hyperpolarized 15N NMR Signal in Blood Plasma. ACS physical chemistry Au, 3(2), 167-171. https://doi.org/10.1021/acsphyschemau.2c00059
dc.identifier.issn2694-2445
dc.identifier.issue2
dc.identifier.urihttps://hdl.handle.net/20.500.12503/32341
dc.identifier.volume3
dc.publisherAmerican Chemical Society
dc.relation.urihttps://doi.org/10.1021/acsphyschemau.2c00059
dc.rights.holder© 2022 The Authors.
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceACS Physical Chemistry Au
dc.titleCompetitive Displacement Restores the Hyperpolarized (15)N NMR Signal in Blood Plasma
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dc.type.materialArticle

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