Comparison of Deparaffinization Methods with DNA Quantification

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2016-08-01

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Leonard, Raechal P.

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Abstract

Formalin-fixed, paraffin embedded (FFPE) tissue samples are often the only sample type available for testing in pathological and clinical fields. Obtaining usable DNA from FFPE samples is difficult due to the formalin fixation method. Formaldehyde, a large component of formalin, causes DNA-protein cross-linking as well as other issues that must be overcome to obtain useable DNA. Many methods for deparaffinization exist to reduce the issues that arise when working with FFPE samples. This project focuses on comparing two methods of deparaffinization: a method described by Bosso and Al-Mulla and a method used by the University of North Texas Center For Human Identification (UNTCHI), to determine if there is a significant difference in DNA obtained from FFPE samples.

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