Comparison of Deparaffinization Methods with DNA Quantification

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dc.contributor.advisorWarren, Joseph E.
dc.contributor.committeeMemberLaRue, Bobby L.
dc.contributor.committeeMemberHodge, Lisa M.
dc.creatorLeonard, Raechal P.
dc.date.accessioned2019-08-22T19:36:05Z
dc.date.available2019-08-22T19:36:05Z
dc.date.issued2016-08-01
dc.date.submitted2016-08-05T09:06:06-07:00
dc.description.abstractFormalin-fixed, paraffin embedded (FFPE) tissue samples are often the only sample type available for testing in pathological and clinical fields. Obtaining usable DNA from FFPE samples is difficult due to the formalin fixation method. Formaldehyde, a large component of formalin, causes DNA-protein cross-linking as well as other issues that must be overcome to obtain useable DNA. Many methods for deparaffinization exist to reduce the issues that arise when working with FFPE samples. This project focuses on comparing two methods of deparaffinization: a method described by Bosso and Al-Mulla and a method used by the University of North Texas Center For Human Identification (UNTCHI), to determine if there is a significant difference in DNA obtained from FFPE samples.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/26380
dc.language.isoen
dc.provenance.legacyDownloads159
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectFFPE
dc.subjectdeparaffinization
dc.subjectDNA quantification
dc.subjecthuman liver disease
dc.titleComparison of Deparaffinization Methods with DNA Quantification
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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