Beta Testing and the Population Genetics of Promega's Prototype PowerPlex Y Kit

Simple item page
dc.contributor.advisorJoseph Warren
dc.contributor.committeeMemberJohn Planz
dc.contributor.committeeMemberArthur Eisenberg
dc.creatorKirkendoll, Ross A.
dc.date.accessioned2019-08-22T21:49:08Z
dc.date.available2019-08-22T21:49:08Z
dc.date.issued2004-08-01
dc.date.submitted2014-05-30T08:00:39-07:00
dc.description.abstractDevelopmental validation is typically done by the manufacturer of the technique or technology. According to National Standards, the manufacturer must test for human specificity to ensure compliance with standards. In addition, the PowerPlex Y kit must be shown to have male specificity because all of the loci are located on the Y-chromosome. Other necessary studies include mixture both male/female and male/male mixture studies, stability studies to show stability in the presence of environmental insults, and the focus of this study the construction of a popular database. In order to satisfy both the requirement of the National Standards and the scrutiny of the legal system, Promega Corporation assembled a collaboration of different laboratories to assist with the developmental validation of the PowerPlex Y Kit. This project was a small part of that collaboration. The DNA Identify Laboratory was chosen by Promega to assist with the construction of a population database because of the number of samples available and the need for confirmed father/son pairs. The objectives of the study were to type ~200 father/son pairs from each of the Caucasian and African American races, and then determine the haplotype frequencies, haplotype diversities, and mutation rates for each race.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29605
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectComputational Biology
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Genetics and Genomics
dc.subjectDevelopmental validation
dc.subjectnational standards
dc.subjectmanufacturer
dc.subjectcompliance
dc.subjectPowerPlex Y kit
dc.subjectY-chromosome
dc.subjectmixture study
dc.subjectfather son pairs
dc.subjecthaplotype frequencies
dc.subjecthaplotype diversities
dc.subjectmutation rates
dc.subjectPromega Corporation
dc.subjectdatabase
dc.titleBeta Testing and the Population Genetics of Promega's Prototype PowerPlex Y Kit
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineCell Biology and Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Kirkendoll_BetaTestingAndThe.pdf
Size:
10.88 MB
Format:
Adobe Portable Document Format
Download

Collections

School of Biomedical Sciences
Theses and Dissertations