Resistance of Bacillus Subtilis Spores Lacking Either Nucleotide Excision Repair or Spore Photoproduct Lyase to Ultraviolet (UV) Radiation from Artificial or Natural Sources

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dc.contributor.advisorTony Romeo
dc.contributor.committeeMemberMing-Chi Wu
dc.contributor.committeeMemberWayne L. Nicholson
dc.creatorXue, Yaming
dc.date.accessioned2019-08-22T21:16:26Z
dc.date.available2019-08-22T21:16:26Z
dc.date.issued1996-06-01
dc.date.submitted2014-02-05T06:30:05-08:00
dc.description.abstractXue, Yaming, Resistance of Bacillus subtilis spores lacking either nucleotide excision repair or spore photoproduct lyase to ultraviolet (UV) radiation from artificial or natural sources. Master of Science (Biomedical Sciences), June 1996, pp., 4 tables, 12 illustrations, references, 38 titles. Exposure of bacterial spores to UV radiation causes the accumulation of a unique pyrimidine dimer in the DNA, “spore photoproduct” (SP). In Bacillus subtilis, two distinct DNA repair pathways are used for removal of SP: general nucleotide excision repair (the uvr parthway), or the SP-specific enzyme SP lyase (the spl pathway). Spores of four strains of Bacillus subtilis differing in their repair capabilities were irradiated under either artificial or solar UV. To determine the biologically-relevant cumulative UV dose under each irradiation condition, a sporocidal dosimeter was constructed. The results showed: (i) Both uvr and spl pathways contributed to the survival of spores under all tested conditions. The spl pathway was more efficient than uvr pathway in repairing the DNA damage caused by UV-C and solar UV-A, but no significant difference was noted in repairing DNA damage caused by UV-B or full-spectrum solar UV. (ii) Exposure of spores to solar UV can cause cellular lethal damage which is reparable by neither repair pathway.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29206
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectBacteria
dc.subjectBacteriology
dc.subjectBiology
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectMicrobiology
dc.subjectOrganismal Biological Physiology
dc.subjectOrganisms
dc.subjectOther Microbiology
dc.subjectResistance
dc.subjectbacillus subtilis spores
dc.subjectnucleotide excision repair
dc.subjectspore photoproduct
dc.subjectUV radiation
dc.subjectDNA repair pathways
dc.subjectspl pathway
dc.subjectuvr pathway
dc.subjectUV-A
dc.subjectUV-B
dc.subjectUV-C
dc.subjectsolar UV
dc.subjectphotoproduct lyase
dc.titleResistance of Bacillus Subtilis Spores Lacking Either Nucleotide Excision Repair or Spore Photoproduct Lyase to Ultraviolet (UV) Radiation from Artificial or Natural Sources
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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