Evaluation or the Gold-Plated Silver Sample Block on the GeneAmp PCR System 9700 Thermal Cycler

Simple item page
dc.contributor.advisorArthur Eisenberg
dc.contributor.committeeMemberJoseph Warren
dc.contributor.committeeMemberJohn Planz
dc.creatorMoore, Melody Ann
dc.date.accessioned2019-08-22T21:42:26Z
dc.date.available2019-08-22T21:42:26Z
dc.date.issued2003-05-01
dc.date.submitted2014-03-18T14:02:27-07:00
dc.description.abstractMoore, Melody Ann. Evaluation of the Gold-plated Silver Sample Block on the GeneAmp PCR System 9700 Thermal Cycler. Master of Science (Forensic Genetics), May 2003, 49 pages, 9 figures, 3 tables, 2 appendices, 24 references. The GeneAmp PRC System 9700 Thermal Cycler has been introduced with interchangeable silver and gold-plated silver sample blocks. To validate the new gold-plated silver sample block on the System 9700, amplifications were performed on both the gold-plated silver and the previously validated silver sample blocks. PCR amplifications using the AmpFLSTR Profiler Plus ID COfiler, Identifiler and SGM Plus typing kits (Applied Biosystems, Forster City, CA) were performed. Electrophoretic characteristics such as allele concordance, peak heights, and peak height ratios were used to discern any differences in the amplification capabilities of the two sample blocks. The results demonstrate that the PCR reactions on both silver and gold-plated silver blocks are equivalent. In addition, the data obtained (allele cells, peak heights, peak height ratios) from both Macintosh and Windows NT platforms were identical for each amplification kit, indicating concordance between the software packages of each operating system. The results of this study demonstrate the interchangeability of the silver and gold-plated sample blocks.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29526
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectEquipment and Supplies
dc.subjectGenetic Processes
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectMolecular Genetics
dc.subjectOther Genetics and Genomics
dc.subjectGold-plated silver sample block
dc.subjectGeneAmp PCR System
dc.subject9700 Thermal Cycler
dc.subjectinterchangeable
dc.subjectamplifications
dc.subjectelectrophoretic characteristics
dc.subjectallele concordance
dc.subjectpeak heights
dc.subjectpeak height ratio
dc.subjectsoftware packages
dc.subjectoperating systems
dc.subjectinterchangeability
dc.subjectMacintosh
dc.subjectWindows NT platforms
dc.titleEvaluation or the Gold-Plated Silver Sample Block on the GeneAmp PCR System 9700 Thermal Cycler
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Moore_EvaluationOfTheGold.pdf
Size:
20.09 MB
Format:
Adobe Portable Document Format
Download

Collections

School of Biomedical Sciences
Theses and Dissertations