Characterization of Protein Kinase C in Cisplatin Sensitive and Resistant Human Cervical Cancer HeLa Cells

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dc.contributor.advisorBasu, Alakananda
dc.contributor.committeeMemberSimecka, Jerry
dc.contributor.committeeMemberDimitrijevich, Dan
dc.creatorMohanty, Sanghamitra
dc.date.accessioned2019-08-22T19:31:26Z
dc.date.available2019-08-22T19:31:26Z
dc.date.issued2000-12-01
dc.date.submitted2014-03-18T14:12:05-07:00
dc.description.abstractMohanty, S., Characterization of protein kinase C in cisplatin sensitive and resistant human cervical cancer HeLa cells. Master of Science (Microbiology and Immunology), December, 2000. 37 pp., 11 illustrations, bibliography, 27 titles. Signal transduction plays a crucial role in carcinogenesis. A defect in signaling, by evading cell death or promoting cell proliferation, may result in neoplastic transformation or protection of cells from the cytotoxicity of anticancer drugs. Therefore, in order to understand the complex mechanism of drug resistance, it is relevant to probe into the important signal transduction pathways. Protein kinase C, a key signal transducer, influences cisplatin sensitivity in many cell lines. We examined whether or not the PKC signal transduction pathway is affected during development of resistance to cisplatin by tumor cells. PKC activators increased cisplatin sensitivity in both parental and cisplatin-resistant cells. Western blot analysis showed a slight decrease in cPKCα and nPKCε, an evaluation in nPKCδ and no change in the abundance of PKCϚ in HeLa/CP cells compared to HeLa cells. Though TPA-induced translocation of PKC isoforms was identical in both cell lines, down regulation of PKCδ was defective in resistant cells. Therefore, a deregulation in PKCδ was associated with cisplatin resistance.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/26092
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCancer Biology
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectCellular and Molecular Physiology
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedicine and Health Sciences
dc.subjectOncology
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Pharmacy and Pharmaceutical Sciences
dc.subjectPharmacology
dc.subjectPharmacy and Pharmaceutical Sciences
dc.subjectToxicology
dc.subjectCharacterization
dc.subjectprotein kinase C
dc.subjectcisplatin sensitive
dc.subjectcisplatin resistant
dc.subjecthuman cervical cancer
dc.subjectHeLa cells
dc.subjectsignal transduction
dc.subjectcarcinogenesis
dc.subjectcell death
dc.subjectcell proliferation
dc.subjectanticancer drugs
dc.subjectcytotoxicity
dc.subjectPKC
dc.titleCharacterization of Protein Kinase C in Cisplatin Sensitive and Resistant Human Cervical Cancer HeLa Cells
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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