Sniffer cells for the detection of neural Angiotensin II in vitro

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dc.creatorFarmer, George E.
dc.creatorAmune, Anna
dc.creatorBachelor, Martha E.
dc.creatorDuong, Phong
dc.creatorYuan, Joseph P.
dc.creatorCunningham, J. Thomas
dc.creator.orcid0000-0001-8859-9481 (Cunningham, J. Thomas)
dc.creator.orcid0000-0002-6099-0080 (Farmer, George E.)
dc.date.accessioned2022-09-16T18:05:03Z
dc.date.available2022-09-16T18:05:03Z
dc.date.issued2019-06-19
dc.description.abstractNeuropeptide release in the brain has traditionally been difficult to observe. Existing methods lack temporal and spatial resolution that is consistent with the function and size of neurons. We use cultured "sniffer cells" to improve the temporal and spatial resolution of observing neuropeptide release. Sniffer cells were created by stably transfecting Chinese Hamster Ovary (CHO) cells with plasmids encoding the rat angiotensin type 1a receptor and a genetically encoded Ca2+ sensor. Isolated, cultured sniffer cells showed dose-dependent increases in fluorescence in response to exogenously applied angiotensin II and III, but not other common neurotransmitters. Sniffer cells placed on the median preoptic nucleus (a presumptive site of angiotensin release) displayed spontaneous activity and evoked responses to either electrical or optogenetic stimulation of the subfornical organ. Stable sniffer cell lines could be a viable method for detecting neuropeptide release in vitro, while still being able to distinguish differences in neuropeptide concentration.
dc.description.sponsorshipThis work was supported by the National Institutes of Health (P01 HL088052, R01 HL119458, R01 HL142341).
dc.identifier.citationFarmer, G. E., Amune, A., Bachelor, M. E., Duong, P., Yuan, J. P., & Cunningham, J. T. (2019). Sniffer cells for the detection of neural Angiotensin II in vitro. Scientific reports, 9(1), 8820. https://doi.org/10.1038/s41598-019-45262-4
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31741
dc.identifier.volume9
dc.publisherSpringer Nature
dc.relation.urihttps://doi.org/10.1038/s41598-019-45262-4
dc.rights.holder© The Author(s) 2019
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientific Reports
dc.subject.meshAngiotensin II / metabolism
dc.subject.meshAnimals
dc.subject.meshBrain / metabolism
dc.subject.meshCHO Cells
dc.subject.meshCricetinae
dc.subject.meshCricetulus
dc.subject.meshFluorescence
dc.subject.meshMale
dc.subject.meshNeurons / metabolism
dc.subject.meshOptogenetics
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshSubstances
dc.titleSniffer cells for the detection of neural Angiotensin II in vitro
dc.typeArticle
dc.type.materialtext

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