Validation of Bodeplex 3 for Typing Small Amplicons and Low Copy Number DNA
dc.contributor.advisor | Arthur Eisenberg | |
dc.contributor.committeeMember | John Planz | |
dc.contributor.committeeMember | Joseph Warren | |
dc.creator | Chahrour, Maria H. | |
dc.date.accessioned | 2019-08-22T21:27:00Z | |
dc.date.available | 2019-08-22T21:27:00Z | |
dc.date.issued | 2003-07-01 | |
dc.date.submitted | 2014-05-02T09:00:05-07:00 | |
dc.description.abstract | Chahrour, Maria H., Validation of BodePlex3 for typing small amplicons and low copy number DNA. Master of Science (Forensice Genetics), July, 2003. 76 pp., 14 tables, 6 figures, references, 25 titles. The BodePlex 3 multiplex is a sensitive mini-STR typing system for analyzing highly degraded and low copy number (LCN) DNA. It allows for the typing of smaller amplicons and decreased quantities of DNA template, thus enhancing the sensitivity of STR analysis of compromised samples. The present study validated BodePlex 3 multiplex system on the ABI PRISM 3100 Genetic Analyzer to be used at The Bode Technology Group for forensic DNA analysis of small amplicons and LCN DNA. Validation experiments were performed according to the DNA Advisory Board (DAB) guidelines. Performance of the multiplex was accurate, reliable and reproducible. The results indicated that the typing system is highly specific for human DNA, sensitive for detecting profiles of LCN DNA, and is capable of resolving mixtures to a certain extent. In addition, this project outlined possible limitations that must be considered for successful use and interpretation of BodePlex 3 DNA profiling results. | |
dc.format.mimetype | application/pdf | |
dc.identifier.uri | https://hdl.handle.net/20.500.12503/29341 | |
dc.language.iso | en | |
dc.provenance.legacyDownloads | 0 | |
dc.subject | Cell and Developmental Biology | |
dc.subject | Computational Biology | |
dc.subject | Equipment and Supplies | |
dc.subject | Forensic Science and Technology | |
dc.subject | Genetics | |
dc.subject | Genetics and Genomics | |
dc.subject | Genetic Structures | |
dc.subject | Health Information Technology | |
dc.subject | Investigative Techniques | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.subject | Other Genetics and Genomics | |
dc.subject | Validation | |
dc.subject | BodePlex3 | |
dc.subject | small amplicons | |
dc.subject | low copy number DNA | |
dc.subject | highly degraded DNA | |
dc.subject | STR analysis | |
dc.subject | compromised samples | |
dc.subject | ABI PRISM 3100 Genetic Analyzer | |
dc.subject | DNA Advisory Board guidelines | |
dc.title | Validation of Bodeplex 3 for Typing Small Amplicons and Low Copy Number DNA | |
dc.type | Professional Report | |
dc.type.material | text | |
thesis.degree.department | Graduate School of Biomedical Sciences | |
thesis.degree.discipline | Cell Biology and Genetics | |
thesis.degree.grantor | University of North Texas Health Science Center at Fort Worth | |
thesis.degree.name | Master of Science |
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