Validation of Bodeplex 3 for Typing Small Amplicons and Low Copy Number DNA

dc.contributor.advisorArthur Eisenberg
dc.contributor.committeeMemberJohn Planz
dc.contributor.committeeMemberJoseph Warren
dc.creatorChahrour, Maria H.
dc.description.abstractChahrour, Maria H., Validation of BodePlex3 for typing small amplicons and low copy number DNA. Master of Science (Forensice Genetics), July, 2003. 76 pp., 14 tables, 6 figures, references, 25 titles. The BodePlex 3 multiplex is a sensitive mini-STR typing system for analyzing highly degraded and low copy number (LCN) DNA. It allows for the typing of smaller amplicons and decreased quantities of DNA template, thus enhancing the sensitivity of STR analysis of compromised samples. The present study validated BodePlex 3 multiplex system on the ABI PRISM 3100 Genetic Analyzer to be used at The Bode Technology Group for forensic DNA analysis of small amplicons and LCN DNA. Validation experiments were performed according to the DNA Advisory Board (DAB) guidelines. Performance of the multiplex was accurate, reliable and reproducible. The results indicated that the typing system is highly specific for human DNA, sensitive for detecting profiles of LCN DNA, and is capable of resolving mixtures to a certain extent. In addition, this project outlined possible limitations that must be considered for successful use and interpretation of BodePlex 3 DNA profiling results.
dc.subjectCell and Developmental Biology
dc.subjectComputational Biology
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Genetics and Genomics
dc.subjectsmall amplicons
dc.subjectlow copy number DNA
dc.subjecthighly degraded DNA
dc.subjectSTR analysis
dc.subjectcompromised samples
dc.subjectABI PRISM 3100 Genetic Analyzer
dc.subjectDNA Advisory Board guidelines
dc.titleValidation of Bodeplex 3 for Typing Small Amplicons and Low Copy Number DNA
dc.typeProfessional Report
dc.type.materialtext School of Biomedical Sciences Biology and Genetics of North Texas Health Science Center at Fort Worth of Science


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